Journal: Nature Communications

Article Title: Cross-species identification of genomic drivers of squamous cell carcinoma development across preneoplastic intermediates

doi: 10.1038/ncomms12601

Figure Lengend Snippet: This occurs for both ( a ) upregulated microRNAs/downregulated target mRNAs and for ( b ) downregulated microRNAs/upregulated target mRNAs. The figures only show those microRNAs with q <10 −8 , log 2 -fold change of >1.15, conserved between species. Validation of select microRNA/mRNA pairs using a distinct set of human matched samples demonstrate robustness of the findings. ( c ) miR-21 is upregulated between NS and cuSCC, whereas predicted targets ARHGAP24 and TIMP3 are downregulated. ( d ) miR-31 is upregulated between NS and cuSCC, whereas predicted targets PTPN14 and FAM134B are downregulated.

Article Snippet: Commercially available Taqman (Life Technologies) probes were acquired for human miR-21 (000397), miR-31 (002279), PTPN14 (Hs00193643_m1), FAM134B (Hs00375273_m1), HMG2A (Hs00171569_m1), TIMP3 (Hs00165949_m1) and ARHGAP24 (Hs01097580_m1) and used in qRT-PCR based quantitation of expression in these tissues, as benchmarked to RNU6B (001093, microRNA) and 18S rRNA (Hs99999901, mRNA).

Techniques: Biomarker Discovery

Journal: Bioscience Reports

Article Title: microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer

doi: 10.1042/BSR20193176

Figure Lengend Snippet: ( A ) Prediction algorithms showed that miR-217 targets the binding site of PTPN14 and the binding site mutant. ( B ) Luciferase assay showed miR-217 mimics significantly reduced the luciferase activity in HEK293T cells after cotransfection with the wild-type luciferase reporter vectors (0.8 μg) and miR-217 mimics (100 nM), while the luciferase activity was not changed when the cells were cotransfected with mutant luciferase reporter vectors (0.8 μg) and miR-217 mimics (100 nM). ( C ) Western blotting showed that miR-217 mimics markedly decreased the protein expression of PTPN14. ( D ) Linear regression revealed a strongly negative correlation between the mRNA expressions of PTPN14 and miR-217 in the gastric tumor tissues of the 40 patients; *, P < 0.05 and **, P < 0.01.

Article Snippet: MiR-217 control, mimics and inhibitors were purchased from GenePharm (Shanghai GenePharma Co.Ltd., China), and si-PTPN14 was obtained from RIBOBIO (Guangzhou RIBOBIO Co.Ltd., China).

Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Cotransfection, Western Blot, Expressing

Journal: Bioscience Reports

Article Title: microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer

doi: 10.1042/BSR20193176

Figure Lengend Snippet: ( A ) Western blotting assays real-time PCR showed that the PTPN14 expression was decreased or increased in SGC-7901 cells after transfection with si-PTPN14 (100 nM) or vector PTPN14 (200 ng/ml), respectively. ( B and C ) Bar showed that the migration and invasion of SGC-7901 and BGC-823 cells were reduced or enhanced when the cells were transfected with si-PTPN14 (100 nM) or vector PTPN14 (200 ng/ml). ( D ) Western blotting assays and real-time PCR showed that the PTPN14 expression was decreased or reversed after transfection with miR-217 mimics (100 nM) or cotransfection with miR-217 mimics (100 nM) and vector PTPN14 (200 ng/ml), respectively. ( E and F ) Bar showed that the migration and invasion of SGC-7901 and BGC-823 cells were reduced or reversed when the cells were transfected with miR-217 mimics (100 nM) or cotransfected with miR-217 mimics (100 nM) and vector PTPN14 (200 ng/ml), respectively. *, P < 0.05; **, P < 0.01.

Article Snippet: MiR-217 control, mimics and inhibitors were purchased from GenePharm (Shanghai GenePharma Co.Ltd., China), and si-PTPN14 was obtained from RIBOBIO (Guangzhou RIBOBIO Co.Ltd., China).

Techniques: Western Blot, Real-time Polymerase Chain Reaction, Expressing, Transfection, Plasmid Preparation, Migration, Cotransfection

Journal: Bioscience Reports

Article Title: microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer

doi: 10.1042/BSR20193176

Figure Lengend Snippet: Western blotting assays showed miR-217 mimics markedly promoted the expression of E-cadherin, and decreased the expression of ZEB1, N-cadherin and Vimentin in SGC-7901 cells after transfection with miR-217 mimics (100 nM). When SGC-7901 cells were cotransfected with miR-217 mimics (100 nM) and vector PTPN14 (200 ng/ml), the expressions of E-cadherin, ZEB1, N-cadherin and Vimentin were reversed.

Article Snippet: MiR-217 control, mimics and inhibitors were purchased from GenePharm (Shanghai GenePharma Co.Ltd., China), and si-PTPN14 was obtained from RIBOBIO (Guangzhou RIBOBIO Co.Ltd., China).

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation